Depletion of CD45RA+ T cells: Advantages and disadvantages of different purification methods.

Conclusions CD45RA-depletion from G-CSF mobilized PBSC is feasible as one- and two-step approach and results in sufficient reduction of CD45RA+ T cells as well as B cells, but also to a co-depletion of NK cells. However, by gaining two independent cell products, the two-step approach enables the highest clinical flexibility in regard to individual graft composition with precise dosage of stem cells and T cells.
PMID: 33417916 [PubMed - as supplied by publisher]

Source: Journal of Immunological Methods - January 5, 2021 Category: Allergy & Immunology Authors: Bremm M, Krastel T, Cappel C, Zimmermann O, Pfeffermann LM, Katzki V, Bonig H, Schäfer R, Rettinger E, Merker M, Bremm S, Schaefer K, Klingebiel T, Soerensen J, Bader P, Huenecke S Tags: J Immunol Methods Source Type: research