Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep.

In this study, the applicability of the fusion construct has been further evaluated and optimised for field applicability. The fusion construct used in an ELISA platform projected a relative diagnostic sensitivity and specificity of 98.1% and 95.5% respectively against a pre-established test panel. The rNS1-NS3 ELISA showed substantially good agreement with the commercial BTV antibody detection kit. Finally, the study brings together the diagnostic capability of two NSPs, which can be a handy tool for sero-surveillance of bluetongue.
PMID: 33412173 [PubMed - as supplied by publisher]

Source: Journal of Immunological Methods - January 4, 2021 Category: Allergy & Immunology Authors: Mohanty NN, Hemadri D, Archana M, Shetty N, Subramanyam V, Biswas SK, Chanda MM, Shivachandra SB Tags: J Immunol Methods Source Type: research