Prevalence and phylogenetic analysis of spike gene of porcine epidemic diarrhea virus in Henan province, China in 2015-2019.

Prevalence and phylogenetic analysis of spike gene of porcine epidemic diarrhea virus in Henan province, China in 2015-2019. Infect Genet Evol. 2021 Jan 04;:104709 Authors: Zhang H, Han F, Yan X, Liu L, Shu X, Hu H Abstract Porcine epidemic diarrhea virus (PEDV) causes diarrhea, dehydration and a high morbidity and mortality in piglets. To investigate the prevalence and molecular characteristics of the spike (S) gene of the PEDV strains, 575 faecal and intestinal samples were collected from individual pigs with diarrhea in 18 regions in Henan, China from April 2015 to March 2019. The detection results showed that PEDV infection was high up to 51.65% in Henan pigs. The PEDV positive rate in suckling piglets was the highest (60.47%), and it existed widely both in PEDV-vaccine immunized (25.00%) and non-immunized pigs (62.29%). The complete S gene of twenty-two representative PEDV strains were sequenced and analyzed. Phylogenetic analysis based on the S gene sequences revealed that the sixteen of the sequenced PEDV Henan strains were located in the G2-a clade and more related to the PEDV variant strains. The other six of the sequenced PEDV strains were closely related to S-INDEL strains and grouped within in the G1-b clade. The Recombinant Identification Program (RIP) and Simplot analysis showed PEDV Henan strains were evolved from the epidemic variant strains and there existed potential recombinant points in the S genome. Furthermore, ...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research