Salmonella genomics and population analyses reveal high inter- and intra- serovar diversity in freshwater.

Salmonella genomics and population analyses reveal high inter- and intra- serovar diversity in freshwater. Appl Environ Microbiol. 2021 Jan 04;: Authors: Deaven AM, Ferreira CM, Reed EA, Chen See JR, Lee NA, Almaraz E, Rios PC, Marogi JG, Lamendella R, Zheng J, Bell RL, Shariat NW Abstract Freshwater can support the survival of the enteric pathogen Salmonella, though temporal Salmonella diversity in a large watershed has not been assessed. At 28 locations within the Susquehanna River basin, 10-liter samples were assessed in spring and summer over two years. Salmonella prevalence was 49%, and increased river discharge was the main driver of Salmonella presence. The amplicon-based sequencing tool, CRISPR-SeroSeq, was used to determine serovar population diversity and detected 25 different Salmonella serovars, including up to ten serovars from a single water sample. On average there were three serovars per sample, and 80% of Salmonella-positive samples contained more than one serovar. Serovars Give, Typhimurium, Thompson, and Infantis were identified throughout the watershed and over multiple collections. Seasonal differences were evident: serovar Give was abundant in the spring, while serovar Infantis was more frequently identified in the summer. Eight of the ten serovars most commonly associated with human illness were detected in this study. Crucially, six of these serovars often existed in the background, where they were masked by a...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research