Serodiagnosis of Lyme borreliosis —is IgM in serum more harmful than helpful?

In this study, we evaluated the diagnostic value of IgM analysis in serum and IgM antibody index (AI) in LB diagnosis. This was a retrospective observational study regardingBorrelia-specific antibodies in serum andBorrelia-specific AI in LB investigations being made during 2017 in J önköping County, Sweden. Medical records of 610 patients with detectable anti-Borrelia antibodies in serum (IgM and/or IgG) and 15 patients with elevatedBorrelia-specific AI were retrospectively scrutinized, and the compliance to current European recommendations was assessed. Among the 610 patients, only 30% were tested according to the European recommendations. Within this group of tests taken correctly, 50% of the LB diagnoses in patients with isolated IgM reactivity in serum were retrospectively assessed as incorrect (LB unlikely). Three pediatric patients with clinical and laboratory findings suggestive of Lyme neuroborreliosis (LNB) had elevated IgM AI alone. Serological testing without distinct clinical signs/symptoms consistent with LB contributes to most misdiagnoses. Isolated IgM positivity in serum shows limited clinical value and needs further assessment before being reported by the laboratory. Detection of IgM in combination with IgG antibodies in serum shows no clinical enhancement for correct LB diagnosis compared to isolated IgG positivity. However,Borrelia-specific IgM AI may be important for sensitivity in early LNB.
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research

Related Links:

Could wildfires be depleting the ozone all over again? Staff Writer Paul Voosen talks with host Sarah Crespi about the evidence from the Polarstern research ship for wildfire smoke lofting itself high into the stratosphere, and how it can affect the ozone layer once it gets there. Next, we talk ticks—the ones that bite, take blood, and can leave you with a nasty infection. Andaleeb Sajid, a staff scientist at the National Cancer Institute, joins Sarah to talk about her Science Translational Medicine paper describing an mRNA vaccine intended to reduce the length of tick bites to before the pests can transmit diseases...
Source: Science Magazine Podcast - Category: Science Authors: Source Type: podcasts
In this study, statistical acrylamide-based cationic glycopolymers with glucose or galactose pendant groups were synthesized by reversible addition-fragmentation chain transfer polymerization, and the effects of the saccharide pendant group and cationic monomer loading on polymer cytotoxicity, RNA complexation, and SelK gene knockdown in ISE6 cells were evaluated. All polymers exhibited low cytotoxicity, yet RNA/copolymer complex cell uptake and gene knockdown were highly dependent on the saccharide structure and the N:P (amino to phosphate groups) ratio.PMID:34793129 | DOI:10.1021/acs.biomac.1c00824
Source: Biomacromolecules - Category: Biochemistry Authors: Source Type: research
Cell Host Microbe. 2021 Nov 10;29(11):1599-1601. doi: 10.1016/j.chom.2021.10.007.ABSTRACTLyme disease, which is caused by the spirochete Borrelia burgdorferi, is on the rise. Current treatment relies on broad-spectrum antibiotics that perturb the gut microbiome. In a recent paper in Cell, Leimer et al. demonstrate the utility of a long-forgotten antibiotic, Hygromycin A, as a spirochete-specific antibacterial that is conducive to gut health.PMID:34762823 | DOI:10.1016/j.chom.2021.10.007
Source: Cell Host and Microbe - Category: Microbiology Authors: Source Type: research
J Immunol. 2021 Nov 10:ji2100815. doi: 10.4049/jimmunol.2100815. Online ahead of print.ABSTRACTComplement evasion is a hallmark of extracellular microbial pathogens such as Borrelia burgdorferi, the causative agent of Lyme disease. Lyme disease spirochetes express nearly a dozen outer surface lipoproteins that bind complement components and interfere with their native activities. Among these, BBK32 is unique in its selective inhibition of the classical pathway. BBK32 blocks activation of this pathway by selectively binding and inhibiting the C1r serine protease of the first component of complement, C1. To understand the st...
Source: Journal of Immunology - Category: Allergy & Immunology Authors: Source Type: research
Cell Host Microbe. 2021 Nov 10;29(11):1599-1601. doi: 10.1016/j.chom.2021.10.007.ABSTRACTLyme disease, which is caused by the spirochete Borrelia burgdorferi, is on the rise. Current treatment relies on broad-spectrum antibiotics that perturb the gut microbiome. In a recent paper in Cell, Leimer et al. demonstrate the utility of a long-forgotten antibiotic, Hygromycin A, as a spirochete-specific antibacterial that is conducive to gut health.PMID:34762823 | DOI:10.1016/j.chom.2021.10.007
Source: Cell Host and Microbe - Category: Microbiology Authors: Source Type: research
Updated Date: Tue, 09 Nov 2021 00:00:00 EST
Source: DailyMed Drug Label Updates - Category: Drugs & Pharmacology Source Type: alerts
Updated Date: Tue, 09 Nov 2021 00:00:00 EST
Source: DailyMed Drug Label Updates - Category: Drugs & Pharmacology Source Type: alerts
AbstractThe purpose of this study was to describe the epidemiology of Lyme neuroborreliosis (LNB) in Kalmar County, in southern Sweden, between 2008 and 2019, and to analyse the relationship between the LNB incidence and climate factors. Data containing cerebrospinal fluid (CSF) cell counts and borrelia CSF/serum antibody index results was received from the departments of clinical chemistry and microbiology at Kalmar County hospital. For this study, we defined LNB as a case with a positive borrelia antibody CSF/serum index and CSF leukocytes  >  5 × 106/L. Climate data including mean te...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
ConclusionDespite being rare, spirochetal uveitis can be detrimental for the vision and must always be included in the differential diagnosis.
Source: International Ophthalmology - Category: Opthalmology Source Type: research
In this study, we characterized the YbaB/EbfC family DNA-binding protein in Caulobacter crescentus. C. crescentus has one YbaB/EbfC family gene annotated in the genome (YbaBCc) and it shares 41% sequence identity with YbaB/EbfC family NAPs. Computational modeling revealed tweezer-like structure of YbaBCc, a characteristic of YbaB/EbfC family of NAPs. N-terminal–CFP tagged YbaBCc localized with the nucleoid and is able to compact DNA. Unlike B. burgdorferi EbfC protein, YbaBCc protein is a non-specific DNA-binding protein in C. crescentus. Moreover, YbaBCc shields DNA against enzymatic degradation. Collectively, our f...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
More News: Borrelia | Brain | Infectious Diseases | Laboratory Medicine | Lyme Disease | Microbiology | Neurology | Pediatrics | Study | Sweden Health