Advances allowing feasible pyrG gene editing by a CRISPR-Cas9 system for the edible mushroom Pleurotus eryngii.

In this study, we identified the point-mutated variant of the endogenous sdhB gene (cbxr) as a more stable selection marker than hygromycin B resistance gene (hph) in P. eryngii. Furthermore, using a codon-optimized Cas9, a predicted native U6 promoter-guided sgRNA, as well as through an optimized protoplast transformation system, a highly efficient pyrG gene editing system was established in P. eryngii, that incorporated varied insertions and deletions (indels) by non-homologous end joining (NHEJ) and homology-directed repair (HDR). Findings for a successful targeted gene editing strategy in the edible mushroom P. eryngii may open a new chapter for the improvement of edible mushroom cultivars. PMID: 33400990 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/33400990?dopt=Abstract

Source: Fungal Genetics and Biology - January 2, 2021 Category: Biology Authors: Wang T, Yue S, Jin Y, Wei H, Lu L Tags: Fungal Genet Biol Source Type: research