Development and evaluation of a 3D Printer-based DNA extraction method coupled to loop mediated isothermal amplification (LAMP) for point-of-care diagnosis of congenital Chagas disease in endemic regions

Vertical transmission of T. cruzi is the cause of congenital Chagas disease, a re-emerging infectious disease that affects endemic and non-endemic regions alike. An early diagnosis is crucial because its prompt treatment achieves a high cure rate, precluding evolution to symptomatic chronic Chagas disease. However, early diagnosis involves low sensitive parasitological assays, making necessary serological confirmation after nine months of life. Aiming at implementing early diagnostic strategies suitable for minimally equipped laboratories, a T.

Source: Journal of Molecular Diagnostics - December 29, 2020 Category: Pathology Authors: Diana Patricia Wehrendt, Julio Alonso-Padilla, Bo Liu, Lizeth Rojas Panozo, Silvia Rivera Nina, Lilian Pinto, Daniel Lozano, Albert Picado, Marcelo Abril, Maria Jesus Pinazo, Joaquim Gascon, Faustino Torrico, Season Wong, Alejandro Gabriel Schijman Tags: Technical Advance Source Type: research