In vitro assessment of antimicrobial resistance dissemination dynamics during multidrug resistant bacteria invasion events using a continuous culture device.

In vitro assessment of antimicrobial resistance dissemination dynamics during multidrug resistant bacteria invasion events using a continuous culture device. Appl Environ Microbiol. 2020 Dec 23;: Authors: Li Z, Shi L, Wang B, Wei X, Zhang J, Guo T, Kong J, Wang M, Xu H Abstract Antimicrobial resistant pathogens display significant public health threats by causing difficulties in clinical treatment of bacterial infection. Antimicrobial resistance (AMR) is transmissible between bacteria, significantly increasing the appearance of antimicrobial resistant pathogens, aggravating the AMR problem. In this work, the dissemination dynamics of AMR from invading multidrug resistant (MDR) Escherichia coli to a community of pathogenic Salmonella enterica was investigated using a continuous culture device, and the behaviors of dissemination dynamics under different levels of antibiotic stress were investigated. Three MDR E. coli invasion events were analyzed in this work: MDR E. coli-S. enterica co-colonization, MDR E. coli invasion after antibiotic treatment of S. enterica, and MDR E. coli invasion before antibiotic treatment of S. enterica It was found that both horizontal gene transfer (HGT) and vertical gene transfer (VGT) play significant roles in AMR dissemination, although different processes contribute differently under different circumstances; that environmental levels of antibiotics promote AMR dissemination by enhancing HGT rather than ...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research