Acquired Resistance to Severe Ethanol Stress on Protein Quality Control in Saccharomyces cerevisiae.

Acquired Resistance to Severe Ethanol Stress on Protein Quality Control in Saccharomyces cerevisiae. Appl Environ Microbiol. 2020 Dec 23;: Authors: Yoshida M, Kato S, Fukuda S, Izawa S Abstract Acute severe ethanol stress (10% v/v) damages proteins and causes the intracellular accumulation of insoluble proteins in Saccharomyces cerevisiae On the other hand, a pretreatment with mild stress increases tolerance to subsequent severe stress, which is called acquired stress resistance. It currently remains unclear whether the accumulation of insoluble proteins under severe ethanol stress may be mitigated by increasing protein quality control (PQC) activity in cells pretreated with mild stress. In the present study, we examined the induction of resistance to severe ethanol stress in PQC, and confirmed that a pretreatment with 6% (v/v) ethanol or mild thermal stress at 37°C significantly reduced insoluble protein levels and the aggregation of Lsg1, which is prone to denaturation and aggregation by stress, in yeast cells under 10% (v/v) ethanol stress. The induction of this stress resistance required the new synthesis of proteins; the expression of proteins comprising the bi-chaperone system (Hsp104, Ssa3, and Fes1), Sis1, and Hsp42 was up-regulated during the pretreatment and maintained under subsequent severe ethanol stress. Since the pretreated cells of deficient mutants in the bi-chaperone system (fes1Δhsp104Δ and ssa2Δssa3Δssa4Δ) f...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research