Arsenate induced changes in bacterial metabolite and lipid pools during phosphate stress.

Arsenate induced changes in bacterial metabolite and lipid pools during phosphate stress. Appl Environ Microbiol. 2020 Dec 23;: Authors: Zhuang W, Balasubramanian N, Wang L, Wang Q, McDermott TR, Copié V, Wang G, Bothner B Abstract Agrobacterium tumefaciens GW4 is a heterotrophic arsenite oxidizing bacterium with a high resistance to arsenic toxicity. It is now a model organism for studying the processes of arsenic detoxification and utilization. Previously, we demonstrated that under low phosphate conditions, arsenate (As(V)) could enhance bacterial growth and be incorporated into biomolecules including lipids. While the basic microbial As(V) resistance mechanisms have been characterized, global metabolic responses under low phosphate remain largely unknown. In the present work, the impact of As(V) and low phosphate on intracellular metabolite and lipid profiles of GW4 were quantified using liquid chromatography-mass spectroscopy (LC-MS) in combination with transcriptional assays and the analysis of intracellular ATP and NADH levels. Metabolite profiling revealed that oxidative stress response pathways were altered and suggested an increase in DNA repair. Changes in metabolite levels in the TCA cycle along with increased ATP are consistent with As(V)-enhanced growth of A. tumefaciens GW4. Lipidomics analysis revealed that most glycerophospholipids decreased in abundance when As(V) was available. However, several glycerolipid classe...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research