Role of SOLUBLE GUANYLYL CYCLASE in RENAL AFFERENT AND EFFERENT ARTERIOLES.

Role of SOLUBLE GUANYLYL CYCLASE in RENAL AFFERENT AND EFFERENT ARTERIOLES. Am J Physiol Renal Physiol. 2020 Dec 28;: Authors: Wennysia IC, Zhao L, Schomber T, Braun D, Golz S, Summer H, Benardeau A, Lai EY, Lichtenberger FB, Schubert R, Persson PB, Xu MZ, Patzak A Abstract Renal arteriolar tone depends considerably on the dilatory action of nitric oxide (NO) via activation of soluble guanylyl cyclase (sGC) and cGMP action. NO deficiency and hypoxia/reoxygenation are important pathophysiological factors in the development of acute kidney injury. It is hypothesized that the NO-sGC-cGMP system functions differently in renal afferent (AA) compared to efferent arterioles (EA) and that the sGC activator cinaciguat differentially dilates these arterioles.Experiments were performed in isolated, perfused mouse glomerular arterioles. Hypoxia (0.1 % oxygen) was achieved by using a hypoxia chamber. Phosphodiesterase 5 (PDE5) and sGC subunits were considerably expressed on the mRNA level in AA. PDE5 inhibition with sildenafil, which blocks cGMP degradation, diminished the responses to Ang II bolus application in AA, but not significantly in EA. Vasodilation induced by sildenafil in Ang II-preconstricted vessels was stronger in EA than AA. Cinaciguat, an NO- and heme-independent sGC activator, dilated EA more strongly than AA after L-NAME (NO synthase inhibitor) treatment and preconstriction with Ang II. Cinaciguat-induced dilatation of L‑NAME ...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research