Comparison of seven commercial SARS-CoV-2 nucleic acid detection reagents with pseudovirus as quality control material

The ongoing pandemic of COVID-19 threats the whole world, which catalyzes a variety of SARS-CoV-2 nucleic acid test (NAT) kits. To monitor test quality and evaluate NAT kits, quality control materials that best simulate real clinical samples are needed in this study, the performance of SARS-CoV-2 cell culture supernatant, PCDH-based pseudovirus, and MS2-based pseudovirus as quality control materials was compared. As a result, PCDH-based pseudovirus was more similar in characteristics to SARS-CoV-2 particle, and was more suitable for evaluating SARS-CoV-2 NAT kits than MS2-based pseudovirus.

Source: Journal of Molecular Diagnostics - December 28, 2020 Category: Pathology Authors: Ying Yan, Le Chang, Wenxin Luo, Junyi Liu, Fei Guo, Lunan Wang Tags: Regular Article Source Type: research