LncRNA CASC9 promotes proliferation, migration and apoptosis of hepatocellular carcinoma cells by down-regulating miR-424-5p.

CONCLUSION: CASC9 reduced proliferation, invasion and migration and induced apoptosis in HCC cells by inhibiting miR-424-5p. LncRNAs, long non-coding RNAs; HCC, hepatocellular carcinoma; AFP, alpha-fetoprotein; CASC9, cancer sensitivity 9; ESCC, esophageal squamous cell carcinoma; qRT-PCR, quantitative reverse transcription polymerase chain reaction; ATCC, American Type Culture Collection; DMEM, Dulbecco's Modified Eagle Medium; CCK-8, cell counting kit-8; RIPA, radio-immunoprecipitation assay; SDS-PAGE, sodium dodecylsulphate polyacrylamide gel electrophoresis; PVDF, polyvinylidene fluoride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; siNC, small interfering RNA of negative control; M: miR-424-5p mimic; IC, inhibitor control; I, miR-424-5p inhibitor. PMID: 33346094 [PubMed - as supplied by publisher]
Source: Annals of Hepatology - Category: Gastroenterology Tags: Ann Hepatol Source Type: research