Expression, purification and crystallization of the N-terminal Solanaceae domain of the Sw-5b NLR immune receptor

Plant nucleotide-binding domain and leucine-rich repeat receptors (NLRs) play crucial roles in recognizing pathogen effectors and activating plant immunity. The tomato NLR Sw-5b is a coiled-coil NLR (CC-NLR) immune receptor that confers resistance against tospoviruses, which cause serious economic losses in agronomic crops worldwide. Compared with other CC-NLRs, Sw-5b possesses an extended N-terminal Solanaceae domain (SD). The SD of Sw-5b is critical for recognition of the tospovirus viral movement protein NSm. An SD is also frequently detected in many NLRs from Solanaceae plants. However, no sequences homologous to the SD have been detected in animals or in plants other than Solanaceae. The properties of the SD protein are largely unknown, and thus 3D structural information is vital in order to better understand its role in pathogen perception and the activation of immune receptors. Here, the expression, purification and crystallization of Sw-5b SD (amino acids 1 – 245) are reported. Native and selenomethionine-substituted crystals of the SD protein belonged to space group P3112, with unit-cell parameters a = 81.53, b   =   81.53, c   =   98.44   Å and a = 81.63, b = 81.63, c = 98.80   Å , respectively. This is the first report of a structural study of the noncanonical SD domain of the NLR proteins from Solanaceae plants.

Source: Acta Crystallographica Section F - December 23, 2020 Category: Biochemistry Authors: Li, J. Xin, J. Zhao, X. Zhao, Y. Wang, T. Xing, W. Tao, X. Tags: Sw-5b Solanaceae domain plant immune receptor Tomato spotted wilt orthotospovirus research communications Source Type: research