A rapid and cost-effective gene expression assay for the diagnosis of well-differentiated and dedifferentiated liposarcomas

Histology does not reliably distinguish benign lipomas from atypical lipomatous tumor / well differentiated liposarcoma, nor dedifferentiated liposarcoma from other pleomorphic sarcomas, entities with different prognosis and management. Molecular confirmation of pathognomonic 12q13-15 amplifications leading to overexpression of MDM2 is a diagnostic gold standard. Currently the most commonly used assay for this purpose is Fluorescent In-Situ Hybridization (FISH), but this is labor-intensive. We assessed if newer NanoString-based technology could allow for more rapid and cost-efficient diagnosis of liposarcomas on standard formalin-fixed tissues through gene expression.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Tags: Technical Advance Source Type: research