Lipid peroxidation and decrease on the activities of antioxidant enzymes in experimental infection by Babesia bovis in cattle

Abstract Babesia bovis is one of the causative agents of bovine babesiosis, a disease with high morbidity and mortality in susceptible populations. The aim of this study was to evaluate the occurrence of oxidative stress in cattle experimentally infected with attenuated B. bovis. For that, eight healthy cattle were used and divided into two groups: animals infected with B. bovis (n = 4; group A) and non-infected animals (n = 4; group B). Blood samples of all animals were collected at 0, 7, 11, and 15 days post-infection (DPI) for red blood cells (RBCs) count, and measurement of thiobarbituric acid reactive substance (TBARS) levels in serum (lipid peroxidation) and activity of antioxidant enzymes in whole blood (catalase (CAT) and superoxide dismutase (SOD)). The parasitemia was determined by blood smear evaluation and conventional PCR for B. bovis. Blood smears were negative throughout the experiment; however, infection was confirmed by PCR positive for B. bovis at 15 DPI. A slight reduction on RBC count was observed in cattle of group A at 11 and 15 DPI (P 
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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This study was conducted at various geographic areas of Erbil governorate. A total of 349 blood samples from equine (209 horses, 62 mule, 57 donkey and 21 ponies) were collected to estimate the prevalence rate of Theileria equi and Babesia caballi by using Giemsa stained blood smear and competitive ELISA. Results: The overall prevalence rates were (10.6%) consisting of (8.3%), (1.7%) and (0.6) for T. equi, B. caballi and both infection respectively by Giemsa stained blood smears, and the rate was 38.97%, consisting of 20.9% for T. equi, 11.2% for B. caballi and 6.9% for both infection by cELISA. Seroprevalence rate of ...
Source: Iranian Journal of Parasitology - Category: Parasitology Tags: Iran J Parasitol Source Type: research
Abstract A PCR targeting mitochondrial cytochrome oxidase subunit III (cox3) for molecular detection of Babesia gibsoni infection in dogs has been developed in this study. Fifty blood samples from suspected clinical cases from dogs, brought to the veterinary college clinics, were examined for presence of B. gibsoni using conventional diagnosis by microscopic examination of Giemsa stained thin blood smears. In addition, species specific PCRs targeting ITS-1 region (BgITS-1 PCR) and nested PCR targeting 18S ribosomal RNA gene (Bg18SnPCR) were carried out. A634 bp PCR fragment of B. gibsoni cox3 gene was amplified in...
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research
CONCLUSIONS: Human babesiosis is probably underdiagnosed due to its non-specific symptoms, lack of awareness about the disease, and the difficulty in making a diagnosis. PMID: 31548143 [PubMed - as supplied by publisher]
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research
This study was carried out with samples belonging to 140 horses residing in Spain and showing common clinical signs of EP. A blood smear microscopic examination and a comparison between the different results obtained by competitive Enzyme-Linked Immunosorbent Assay (cELISA), real-time Polymerase Chain Reaction (PCR) and hematological and biochemical (direct and total bilirubin) screening were conducted. EP positivity rates by cELISA and PCR were 50.7% and 42.9%, respectively, whereas only 9% of the horses were positive in the microscopic analysis. A significantly higher number of B. caballi-positive horses were detected by...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Answer toParasite Case of the Week 558:Plasmodium falciparummalaria,>10% parasitemia. NEGATIVE rapid antigen.Sowhy is the rapid antigen test negative???As noted by our readers, there are many possible reasons for apositive blood smear and negative rapid malaria antigen test (RDT). Here are our options, along with the reasons why each is or isn't a likely explanation in this case:This is babesiosis, and not malaria. This is a very important consideration given the morphologic similarities betweenBabesiaspp. andPlasmodium falciparum.However, the moprhologic features in this case are highly consistent withP. falciparu...
Source: Creepy Dreadful Wonderful Parasites - Category: Parasitology Source Type: blogs
Abstract Canine babesiosis (CB) is a significant tick-borne disease caused by intra-erytrocytic protozoa of the genus Babesia (Apicomplexa: Piroplasmida) and is currently emerging or re-emerging in many European countries. Despite the increasing incidence of clinical CB, little is known of the epidemiology of this disease in Romania. Therefore, the present study was conducted to provide epidemiological data on CB in the coastal area of the Black Sea (Dobrogea), southeastern Romania. For this, 306 owned dogs presented during 2016-2018 at a veterinary clinic in the area and clinically suspected for CB were tested fo...
Source: The Journal of Parasitology - Category: Parasitology Authors: Tags: J Parasitol Source Type: research
AbstractThe present study was undertaken on epidemiology and diagnosis of babesiosis in sheep and goats in Bengaluru Urban and Rural districts of Karnataka state from November 2017 to May 2018. Out of 343 (225 sheep and 118 goats) blood smears examined by Giemsa and acridine orange (AO) fluorescent dye staining methods, 3.55 and 4.0 per cent of sheep and 0.84 and 1.69 per cent of goat samples were found positive forBabesia organisms, respectively. The sensitivity and specificity was found to be higher in AO fluorescent dye staining method. In agewise susceptibility, the percent positivity was found to be higher in animals&...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
In this study Anaplasmosis (14%) was highly prevalent during the winter season and Babesiosis (13.73%) was highly prevalent during summer months followed by Anaplasmosis (9.8%) and Theileriosis (7.8%), the lowest prevalence of Trypanosomiasis was observed during the rainy season. The s easonal variation in prevalence of haemoprotozoan disease might be due to influence of climatic factors on density of vector population in that geographical area. Haemogram revealed decreased level of haemoglobulin, packed cell volume and total erythrocyte count. The serum biochemistry revealed elev ated level of liver enzyme Asparate transa...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
Abstract Babesiosis is a worldwide emerging tick-borne disease that is increasing in frequency and geographic range. It imposes a significant health burden, especially on those who are immunocompromised and those who acquire the infection through blood transfusion. Death from babesiosis occurs in up to 20 percent of these groups. Diagnosis is confirmed with identification of typical intraerythrocytic parasites on a thin blood smear or Babesia DNA using PCR. Treatment consists of atovaquone and azithromycin or clindamycin and quinine, and exchange transfusion in severe cases. Personal and communal protective measur...
Source: International Journal for Parasitology - Category: Parasitology Authors: Tags: Int J Parasitol Source Type: research
AbstractA 5  years old crossbred cow was brought to the Veterinary Clinical Complex of Lala Lajpat Rai University of Veterinary and Animal Sciences Hisar with history of progressive weakness, pale mucous membrane, anorexia, high fever (105 °F), tachycardia, laboured breathing and coffee coloured urine. Analy sis of haematological parameters revealed severe anaemia, leucocytopenia, marked poikilocytosis of erythrocytes. Blood smear examination showed presence of signet ring shapedTheileria organisms,Babesia piroplasms and condensed dot forms ofAnaplasma marginale in the stained erythrocytes. Further animal was...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
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