Effect of cyanocobalamin on oocyte maturation, in vitro fertilization, and embryo development in mice.

Effect of cyanocobalamin on oocyte maturation, in vitro fertilization, and embryo development in mice. Zygote. 2020 Dec 17;:1-8 Authors: Rostami T, Fathi F, Assadollahi V, Hosseini J, Khadem Erfan MB, Rashidi A, Amiri G, Banafshi O, Alasvand M Abstract The aim of this study was to investigate the effect of cyanocobalamin supplementation on in vitro maturation (IVM), in vitro fertilization (IVF), and subsequent embryonic development competence to the blastocyst stage, and in vitro development of mouse 2-cell embryos. Cumulus cells were prepared from mouse cumulus-oocyte complexes (COCs) and incubated for 24 h in an in vitro culture (IVC) medium that contained different concentrations of cyanocobalamin (100, 200, 300 or 500 pM). We collected 2-cell embryos from superovulated NMRI mice and cultured them in the same concentrations of cyanocobalamin (100, 200, 300 or 500 pM). After 42 h of IVM, we observed significantly increased oocyte maturation in the 200 pM cyanocobalamin-treated group compared with the control group (P < 0.0001). Mature oocytes cultured in 200 pM cyanocobalamin were fertilized and cultured in IVC medium with cyanocobalamin (100, 200, 300 or 500 pM) during early embryogenesis. The matured oocytes that were cultured in 200 pM cyanocobalamin had significantly higher 2-cell development rates compared with the control oocytes (P < 0.01). Embryos obtained from in vitro mature oocytes and in vivo fertilized oocytes th...
Source: Zygote - Category: Reproduction Medicine Authors: Tags: Zygote Source Type: research