Argentic staining reveals changes in cerebellar tissue organisation by prenatal glucocorticoid administration in rats.

Argentic staining reveals changes in cerebellar tissue organisation by prenatal glucocorticoid administration in rats. Histol Histopathol. 2020 Dec 17;:18291 Authors: Rivas-Manzano P, Ramírez-Escoto MM, De la Rosa-Rugerio C, Rugerio-Vargas C, Ortiz-Hernández R, Torres-Ramírez N Abstract It was almost 150 years ago that Golgi revolutionised histology with silver-based stains. Major advances in knowledge of the nervous system became possible because of silver impregnations. Silver staining combined with classical histological staining, cytochemistry methods, and electron microscopy is useful for studying mechanisms and components at subcellular, cellular, and tissue levels. Despite the advantages of silver staining, its use has decreased over time. The aim of this work was to use argentic staining to study the cerebellar effects of controversial prenatal glucocorticoid (GC) therapy. At postnatal day 12 (P12), the cerebellum of corticosterone (CC)-treated rats impregnated with AgNOR staining exhibited diminished thickness of the external granule layer (EGL) and irregular Purkinje cell arrangement. There was a greater number of nucleoli and nucleolar organiser regions (NORs) in 24% of Purkinje cells. Cerebellar granule neuron progenitor (CGNP) cells of the EGL showed a decrease in cellular density (confirmed by proliferating cell nuclear antigen [PCNA] immunolocalization) and NORs. At postnatal day 6 (P6), the Golgi-Kopsch technique a...
Source: Histology and Histopathology - Category: Cytology Tags: Histol Histopathol Source Type: research