Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells.

Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells. Cell Stem Cell. 2020 Dec 10;: Authors: Stirparo GG, Smith A, Guo G Abstract Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point "mutations" in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero. PMID: 33321074 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/33321074?dopt=Abstract

Source: Cell Stem Cell - December 10, 2020 Category: Stem Cells Authors: Stirparo GG, Smith A, Guo G Tags: Cell Stem Cell Source Type: research