Bovine lactoferrin and lactoferrin peptides affect endometrial and cervical cancer cell lines.
In this study, we explored the effect of bLF, LFchimera and its constituent peptides LFcin17-30 and LFampin265-284 on the viability of cervical (HeLa, SiHa) and endometrial (KLE, HEC-1A) cancer cell lines. Cell proliferation was quantified with an MTT assay; cell morphological changes and damage were determined by Giemsa and phalloidin-TRITC and DAPI staining; and apoptotic and necrotic cells were identified by Alexa Fluor® 488 Annexin V and PI staining. Additionally, the effect of combinations of bLF and LF-peptides with cisplatin was assessed. BLF and LF-peptides inhibited the proliferation of uterine cancer cells and caused cellular morphological changes and damage to cell monolayers. BLF induced apoptosis; LFcin17-30 and LFampin265-284 induced apoptosis and necrosis, and LFchimera induced necrosis. Additionally, bLF and LFchimera showed an additive interaction with cisplatin against uterine cancer cells.
PMID: 33307991 [PubMed - as supplied by publisher]
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Ramírez-Sánchez DA, Arredondo-Beltran IG, Canizalez-Roman A, Flores-Villaseñor H, Nazmi K, Bolscher JGM, Leon-Sicairos N Tags: Biochem Cell Biol Source Type: research
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