Vogt Koyanagi Harada patients show higher frequencies of circulating NKG2Dpos NK and NKT cells.

Vogt Koyanagi Harada patients show higher frequencies of circulating NKG2Dpos NK and NKT cells.
Clin Exp Immunol. 2020 Dec 12;:
Authors: Bonacini M, Cimino L, De Simone L, Bolletta E, Gozzi F, Soriano A, Muratore F, Zerbini A, Fontana L, Salvarani C, Croci S
Abstract
Vogt Koyanagi Harada (VKH) is an autoimmune disease characterized by inflammation in tissues that contain melanocytes. We aimed to increase the knowledge on immunological pathways deregulated in VKH disease. We compared the percentages of circulating NK, NKT and T cells expressing the activatory markers: CD16, CD69, NKG2D, Nkp30, Nkp46, and the inhibitory marker: NKG2A in 10 active VKH patients, 20 control subjects (CTR) and 7 patients with Behçet disease (BD) by flow cytometry. Cytotoxic potential of NK cells was determined through the degranulation marker CD107a expression after contact with K562 cells by flow cytometry. Moreover, plasmatic levels of 27 cytokines were determined with a multiplex bead-based assay. VKH patients showed higher percentages of NKG2Dpos NK and NKT cells versus CTR. The cytotoxic potential of NK cells induced by K562 cells was comparable between VKH patients and CTR. Finally, higher concentrations of IL-4, IL-5, IL-7, IL-17, PDGF-bb were detected in plasma of VKH patients versus CTR. The immune profile of VKH patients was similar to that of BD patients.
PMID: 33314028 [PubMed - as supplied by publisher]

Source: Clinical and Developmental Immunology - December 12, 2020 Category: Allergy & Immunology Authors: Bonacini M, Cimino L, De Simone L, Bolletta E, Gozzi F, Soriano A, Muratore F, Zerbini A, Fontana L, Salvarani C, Croci S Tags: Clin Exp Immunol Source Type: research