Comparison of the analytical performance between the Oncomine Dx Target Test and a conventional single gene test for epidermal growth factor receptor mutation in non ‐small cell lung cancer

ConclusionsThe analytical performance of the Oncomine Dx Target Test analysis forEGFR mutations may not be comparable with conventional single gene tests due to both invalid and false ‐negative results.Key pointsSignificant findings of the study The success rate of the Oncomine Dx Target Test was significantly lower than the PNA ‐LNA PCR clamp test. Among the samples successfully analyzed, four exon 19 deletions were not detected by the Oncomine Dx Target Test.What this study adds The analytical performance of the Oncomine Dx Target Test may not be comparable with conventional single gene tests. We should revise the sampling procedures, and review the sample quality assessment methods, to improve the analytical performance.

https://onlinelibrary.wiley.com/doi/abs/10.1111/1759-7714.13767?af=R

Source: Thoracic Cancer - December 12, 2020 Category: Cancer & Oncology Authors: Tadashi Sakaguchi, Akemi Iketani, Kazuki Furuhashi, Yuki Nakamura, Yuta Suzuki, Kentaro Ito, Kentaro Fujiwara, Yoichi Nishii, Koji Katsuta, Osamu Taguchi, Osamu Hataji Tags: ORIGINAL ARTICLE Source Type: research