Nicotinamide ameliorates energy deficiency and improves retinal function in Cav ‐1‐/‐ mice

In this study, NAM was administrated orally for two weeks in Cav‐1‐/‐ mice before experiments. Oxidative lipidomics was conducted to detect the oxylipin changes, the retinal energy flux was measured by seahorse assay, and the retinal function was assessed by electroretinogram (ERG). Cav ‐1 deficiency induced dysregulation of oxidative lipidomics and reduction in energy consumption/production in the retina by decreasing Na+/K+‐ATPase, oxidative phosphorylation CII, cytochrome c and oxygen consumption rate(OCR). A decrease in Sirt1 was also detected. Therapeutic administration of NAM significantly increased Sirt1 expression and improved energy deficiency by increasing Na+/K+‐ATPase, cytochrome c and OCR. The dysregulation of oxidative lipidomics was partially recovered, and the retinal function was improved as assessed by ERG compared to Cav‐1‐/‐ mice.  Our study demonstrated the dysregulation of oxidative lipidomics in Cav‐1‐/‐ retina and established a link between energy deficiency and retinal function deficits in Cav ‐1‐/‐ mice. Administration of NAM ameliorated energy deficiency, increased the expression of Sirt1 and improved retinal function, which presents a potential therapeutic strategy for Cav ‐1 deficiency‐induced retinal function deficits.
Source: Journal of Neurochemistry - Category: Neuroscience Authors: Tags: ORIGINAL ARTICLE Source Type: research