Role of tesmin expression in non-small cell lung cancer.

Role of tesmin expression in non-small cell lung cancer. Oncol Lett. 2021 Jan;21(1):48 Authors: Grzegrzolka J, Olbromski M, Gomulkiewicz A, Piotrowska A, Glatzel-Plucinska N, Ratajczak K, Sputa-Grzegrzolka P, Rzechonek A, Werynska B, Podhorska-Okolow M, Dziegiel P Abstract Non-small cell lung cancer (NSCLC) is the most commonly diagnosed cancer and the most frequent cause of cancer-associated mortality worldwide. Tesmin (MTL5) is a 60 kDa protein which has cysteine rich motifs, characteristic of metallothioneins. Tesmin expression was first observed in germ cells during spermatogenesis. Increased tesmin expression in NSCLC has been described previously. Minichromosome maintenance proteins (MCMs) serve a critical role in replication and cell cycle progression, i.e. in NSCLC. The aim of the present study was to evaluate the localization and intensity of tesmin, MCM5 and MCM7 protein expression in NSCLC and their association with the clinicopathological data of patients. Archival paraffin blocks of 243 cases of NSCLC and 104 non-cancerous tissue samples from the surgical margin (control) were obtained from patients treated at the Clinic of Thoracic Surgery of Wroclaw Medical University (Wroclaw, Poland) between 2010 and 2016, and were used for tissue microarrays and immunohistochemical (IHC) experiments. Laser capture microdissection was used for the isolation of cancer cells from 36 frozen samples of NSCLC and 8 control samples, and su...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research