Lipid and biodiesel production by cultivation isolated strain Chlorella sorokiniana pa.91 and Chlorella vulgaris in dairy wastewater treatment plant effluents

AbstractThe present study provided a comparison of two species of microalgae growth in dairy wastewater treatment plant effluents. In optimum conditions their operation to biomass production, lipid accumulation and fatty acids methyl ester composition so as to biodiesel production is studied. For the first time, the not sterilized effluents of dairy wastewater treatment plant was used as the culture mediums of native microalgae,Chlorella sorokiniana strain pa.91, and another oneChlorella vulgaris. They were cultured under 5 light intensity levels so as to find optimum conditions to observed high biomass and lipid production. At the optimum light intensity the composition of fatty acids methyl ester in their lipids was analyzed by GC-MS. The light intensity of 7500 Lux was obtained as the optimum for both microalgae to produce high biomass. The biomass productivity ofC. sorokiniana pa.91 andC. vulgaris in preliminary treated effluent at this light intensity was obtained 0.233 and 0.214  g L−1 day−1, respectively. This parameter in secondary treated effluent was achieved 0.185 and 0.166  g L−1 day−1, respectively. Moreover, the highest lipid content of their biomass was observed at the light intensity of 2500 Lux. At this light intensity and at the preliminary effluent the maximum lipid content ofC. sorokiniana pa.91 andC. vulgaris was observed 31% and 34%, respectively and at the secondary one it was obtained 35% and 36.67%, respectively. Based on the results, th...
Source: Journal of Environmental Health Science and Engineering - Category: Environmental Health Source Type: research