The effect of honokiol on ergosterol biosynthesis and vacuole function in Candida albicans.

The effect of honokiol on ergosterol biosynthesis and vacuole function in Candida albicans. J Microbiol Biotechnol. 2020 Nov 27;: Authors: Sun L, Liao K Abstract Ergosterol, an essential constituent of membrane lipids of yeast, is distributed in both the cell membrane and the intracellular endomembrane system such as vacuoles. Honokiol, a major polyphenol isolated from Magnolia officinalis, has been shown to inhibit the growth of Candida albicans. Here, we assessed the effect of honokiol on ergosterol biosynthesis and vacuole function in C. albicans. Honokiol could decrease the ergosterol content and upregulate the expression of genes related with the ergosterol biosynthesis pathway. The exogenous supply of ergosterol attenuated the toxicity of honokiol against C. albicans. Honokiol treatment could induce cytosolic acidification by blocking the activity of the plasma membrane Pma1p H+-ATPase. Furthermore, honokiol caused the abnormal of vacuole morphology and function. Concomitant ergosterol feeding to some extent restored the vacuolar morphology and the function of acidification in cells treated by honokiol. Honokiol also disrupted the intracellular calcium homeostasis. Amiodarone attenuated the antifungal effects of honokiol against C. albicans, probably due to the activation of the calcineurin signalling pathway which is involved in honokiol tolerance. PMID: 33263334 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research