One-step grain pretreatment for ochratoxin A detection based on bipolar electrode-electrochemiluminescence biosensor.

One-step grain pretreatment for ochratoxin A detection based on bipolar electrode-electrochemiluminescence biosensor.
Anal Chim Acta. 2021 Jan 02;1141:83-90
Authors: Lu L, Yuan W, Xiong Q, Wang M, Liu Y, Cao M, Xiong X
Abstract
False positives are common and frequently occurring in detection of ochratoxin A (OTA) due to the complexity of the food matrix. In this paper, a novel bipolar electrode-electrochemiluminescence (BPE-ECL) sensing platform for sensitive OTA detection with one-step grain pretreatment was proposed. The biosensor uses cathode of closed BPE as a functional sensing interface and anode as a signal collection interface. On the functional sensing interface, the horseradish peroxidase (HRP) catalyze the polymerization of aniline to form polyaniline (PANI) on nucleic acid backbone which is supplied by DNA tetrahedron-structured aptamer (DTA) and hybrid chain reaction (HCR). In the presence of OTA, PANI is formed and can cause the change of ECL and luminescence voltage of the anode of BPE. On the signal collection interface, the Ru(bpy)32+/TPA system is used as ECL light output. In this way, the analyte does not need to participate the ECL reaction of the anode, which avoids direct contact of photoactive molecules with complex reaction systems and greatly reduce the influence of complex food matrix on signal acquisition. The accuracy of the BPE-ECL biosensor (one-step grain pretreatment) was similar with high performance ...

Source: Analytica Chimica Acta - December 1, 2020 Category: Chemistry Authors: Lu L, Yuan W, Xiong Q, Wang M, Liu Y, Cao M, Xiong X Tags: Anal Chim Acta Source Type: research