Leaf extracts of Campomanesia xanthocarpa positively regulates atherosclerotic-related protein expression.

Leaf extracts of Campomanesia xanthocarpa positively regulates atherosclerotic-related protein expression.
An Acad Bras Cienc. 2020;92(4):e20191486
Authors: Cunha EBB, Silva NFD, Lima J, Serrato JA, Aita CAM, Herai RH
Abstract
Atherosclerosis is caused by a monocyte-mediated inflammatory process that, in turn, is stimulated by cytokines and adhesion molecules. Monocytes are then differentiated into macrophages, leading to the formation of arterial atherosclerotic plaques. Recently, guavirova leaf extracts from Campomanesia xanthocarpa (EG) have shown potential effects on the treatment of plaque formation by reducing cholesterol, LDL levels and serum oxidative stress. We evaluated the effect of EG on the viability of human monocytic and endothelial cell lines at three time points (24, 48 and 72 hours) and whether it can modulate the migration and in vitro expression of CD14, PECAM-1, ICAM-1, HLA-DR and CD105. Cell viability was affected only at higher concentrations and times. We observed decreased ICAM-1 expression in cells treated with 50 μg/ml EG and CD14 expression with IFN-γ and without IFN-γ. CD14 also decreased endothelial cell expression in the presence of IFN-γ and GE. We also found decreased expression of PECAM-1 when treated with EG and IFN-γ. In addition, EG-treated endothelial cells showed higher migration than the control group. Reduced expression of these markers and increased migration may lead to decreased cytoki...

Source: An Acad Bras Cienc - November 26, 2020 Category: Science Authors: Cunha EBB, Silva NFD, Lima J, Serrato JA, Aita CAM, Herai RH Tags: An Acad Bras Cienc Source Type: research