G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures.

G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures. Methods Mol Biol. 2021;2239:251-268 Authors: McIntire E, Leonhard K, Taapken S, Larson AL Abstract Acquired chromosomal abnormalities may occur during the reprogramming and culture of human pluripotent stem cells (hPSCs). Therefore, it is required that regular testing of genetic integrity be conducted. G-banded karyotyping is a widely used genetic assay that is capable of detecting chromosomal abnormalities. Karyotyping of hPSC cultures can be a challenging undertaking for inexperienced investigators; here, we provide detailed procedures for karyotyping, including sample preparation and analysis, as well as the interpretation of hPSC karyotype results. PMID: 33226624 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research