Molecular complexes of calf thymus DNA with various bioactive compounds: Formation and characterization.

Molecular complexes of calf thymus DNA with various bioactive compounds: Formation and characterization.
Int J Biol Macromol. 2020 Nov 20;:
Authors: Ji C, Yin X, Duan H, Liang L
Abstract
The interaction between biomacromolecules and ligands has attracted great interest because of their biological properties. Calf thymus DNA (ctDNA) can interact with bioactive compounds to form complexes. Here, ctDNA-ligand complexes were studied using fluorescence, absorption, and infrared spectroscopy, circular dichroism, ABTS assay and competitive displacement. The binding constants of bioactive compounds at the intercalative site of ctDNA ranked in order kaempferol > apigenin > quercetin > curcumin > riboflavin, while the binding constants at minor groove sites ranked quercetin > kaempferol > naringenin ~ apigenin > hesperetin > curcumin ~ resveratrol ~ riboflavin > caffeic acid. CtDNA maintained stable B-form with an enhancement of base stacking and a decrease of right-handed helicity in the presence of these bioactive compounds, except for hesperetin and caffeic acid. Bioactive compounds preferentially bound to guanine bases and tended to transfer into a more hydrophobic environment upon complexation with ctDNA. The DNA complexation did not affect the ABTS·+ scavenging capacity of quercetin, kaempferol, resveratrol and apigenin but increased the ones of naringenin, caffeic acid, curcumin, hesperetin and riboflavin. Th...

Source: International Journal of Biological Macromolecules - November 20, 2020 Category: Biochemistry Authors: Ji C, Yin X, Duan H, Liang L Tags: Int J Biol Macromol Source Type: research