Inhibition of HIF-1 α/EP4 axis by hyaluronate-trimethyl chitosan-SPION nanoparticles markedly suppresses the growth and development of cancer cells.

In this study, we used hyaluronate (HA), and trimethyl chitosan (TMC) recoated superparamagnetic iron oxide nanoparticles (SPIONs) loaded with HIF-1α-silencing siRNA and the EP4 antagonist (E7046) to treat cancer cells and assessed the effect of combination therapy on cancer progression. The results showed that optimum physicochemical characteristics of NPs (size 126.9 nm, zeta potential 27 mV, PDI < 0.2) and linkage of HA with CD44 molecules overexpressed on cancer cells could deliver siRNAs to cancer cells and significantly suppress the HIF-1α in them. Combination therapy of cancer cells by using HIF-1α siRNA-loaded SPION-TMC-HA NPs and E7046 also prevent proliferation, migration, invasion, angiogenesis, and colony formation of the cancer cells, remarkably.
PMID: 33227333 [PubMed - as supplied by publisher]

Source: International Journal of Biological Macromolecules - November 20, 2020 Category: Biochemistry Authors: Karpisheh V, Afjedi JF, Afjadi MN, Haeri MS, Sough TSA, Asl SH, Edalati M, Atyabi F, Masjedi A, Hajizadeh F, Izadi S, Tekie FSM, Hajiramezanali M, Sojoodi M, Jadidi-Niaragh F Tags: Int J Biol Macromol Source Type: research