In ovo feeding of vitamin C regulates splenic development through purine nucleotide metabolism and induction of apoptosis in broiler chickens.

In ovo feeding of vitamin C regulates splenic development through purine nucleotide metabolism and induction of apoptosis in broiler chickens. Br J Nutr. 2020 Nov 23;:1-34 Authors: Zhu L, Wang J, Li Z, Ma H, Zhu Y, Yang X, Yang X Abstract The nutrition condition of earlier life has a long-term influence on health of its later period. In order to the explore effects of in ovo feeding of vitamin C on splenic development, splenic metabolism and apoptosis were detected in embryo, adult chickens and in vitro. A total of 360 fertile eggs were selected and randomly assigned to 2 treatments: the control group (CON) and vitamin C group (VC) were injected with saline and vitamin C on embryonic day 11, respectively. Functional enrichment of differentially expressed genes by transcriptome on embryonic day 19 suggested that purine nucleotide metabolism might be a potential pathway for the in ovo feeding of vitamin C to regulate spleen development. Additionally, the in ovo feeding of vitamin C significantly increased splenic vitamin C content on post-hatch day 21. Meanwhile, the splenic expression of adenosine deaminase, serine/threonine kinase 1 and proliferating cell nuclear antigen were down-regulated, whereas the expression of cysteinyl aspartate specific proteinase 9 was up-regulated in VC group. On post-hatch day 42, the in ovo feeding of vitamin C significantly down-regulated the splenic expression of B-cell lymphoma 2, and increased the mR...
Source: The British Journal of Nutrition - Category: Nutrition Authors: Tags: Br J Nutr Source Type: research