Tomato thymidine kinase-based suicide gene therapy for malignant glioma-an alternative for Herpes Simplex virus-1 thymidine kinase.

Tomato thymidine kinase-based suicide gene therapy for malignant glioma-an alternative for Herpes Simplex virus-1 thymidine kinase. Cancer Gene Ther. 2015 Jan 23; Authors: Stedt H, Samaranayake H, Kurkipuro J, Wirth G, Christiansen LS, Vuorio T, Määttä AM, Piškur J, Ylä-Herttuala S Abstract Malignant gliomas (MGs) are the most common malignant primary brain tumors with a short life estimate accompanied by a marked reduction in the quality of life. Herpes Simplex virus-1 thymidine kinase ganciclovir (HSV-TK/GCV) system is the best characterized enzyme prodrug therapy in use. However, lipophobicity of GCV and low enzymatic activity of HSV-TK reduce the treatment efficacy. Tomato TK (ToTK) has shown high activity in combination with its specific substrate azidothymidine (AZT). The aim of this study was to evaluate whether ToTK/AZT could be used as an alternative to HSV-TK/GCV therapy. Both treatments demonstrated cytotoxicity in human MG cells in vitro. In vivo, both treatments decreased tumor growth and tumors were smaller in comparison with controls in mouse orthotopic MG model. Survival of ToTK/AZT-treated mice was significantly increased compared with control mice (*P
Source: Herpes - Category: Infectious Diseases Authors: Tags: Cancer Gene Ther Source Type: research

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Source: Acta Clinica Croatica - Category: General Medicine Tags: Acta Clin Croat Source Type: research
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Source: Acta Clinica Croatica - Category: General Medicine Tags: Acta Clin Croat Source Type: research
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Source: Acta Clinica Croatica - Category: General Medicine Tags: Acta Clin Croat Source Type: research
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Source: Acta Clinica Croatica - Category: General Medicine Tags: Acta Clin Croat Source Type: research
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Source: LWT Food Science and Technology - Category: Food Science Source Type: research
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Source: Gut Microbes - Category: Microbiology Authors: Source Type: research
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Source: Gut Microbes - Category: Microbiology Authors: Source Type: research
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Source: Neurological Research - Category: Neurology Tags: Neurol Res Source Type: research
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Source: Journal of Biosciences - Category: Biomedical Science Authors: Tags: J Biosci Source Type: research
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Source: Journal of Biosciences - Category: Biomedical Science Authors: Tags: J Biosci Source Type: research
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