Structural studies of immunomodulatory (1  → 3)-, (1 → 4)-α glucan from an edible mushroom Polyporus grammocephalus.

Structural studies of immunomodulatory (1 → 3)-, (1 → 4)-α glucan from an edible mushroom Polyporus grammocephalus.
Int J Biol Macromol. 2020 Nov 18;:
Authors: Patra S, Maity P, Chakraborty I, Sen IK, Ghosh D, Rout D, Bhanja SK
Abstract
A water soluble polysaccharide (PGPS) with molecular weight ~ 1.4 × 105 Da was isolated by alkali treatment from an edible mushroom Polyporus grammocephalus and purified by gel chromatography using sepharose-6B column. Monosaccharide analysis revealed that PGPS was made up of glucose only. PGPS contained (1 → 3)-α-D-Glcp and (1 → 4)-α-D-Glcp moieties in a molar ratio of nearly 1:2. Through a series of chemical and spectroscopic (1D/2D NMR) investigations, the repeating unit of the glucan was established as: →3)-α-D-Glcp(1 → [4)-α-D-Glcp(1]2→ This α-glucan was observed to stimulate some prime components of immune system, namely, macrophages, splenocytes, and thymocytes.
PMID: 33220371 [PubMed - as supplied by publisher]

Source: International Journal of Biological Macromolecules - November 18, 2020 Category: Biochemistry Authors: Patra S, Maity P, Chakraborty I, Sen IK, Ghosh D, Rout D, Bhanja SK Tags: Int J Biol Macromol Source Type: research