Volatile metabolic markers for monitoring Pectobacterium carotovorum subsp. carotovorum using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry.

In this study, we used headspace solid-phase microextraction followed by gas chromatography coupled with mass spectrometry (HS-SPME-GC-MS) to identify volatile compounds (VCs) in PCC-inoculated cabbage at different storage temperatures. HS-SPME-GC-MS allowed for recognition of extracellular metabolites in PCC-infected cabbages by identifying specific volatile metabolic markers. 4-Ethyl-5-methylthiazole and 3-butenyl isothiocyanate were identified as markers of fresh cabbages, whereas 2,3-butanediol and ethyl acetate were identified as markers of soft rot in PCC-infected cabbages. These analytical results demonstrate a suitable approach for establishing non-destructive plant pathogen-diagnosis techniques as alternatives to standard methods, within the framework of the development of rapid and efficient analytical techniques for monitoring plant-borne bacterial pathogens. These techniques also demonstrate promising applications for the freshness management and quality control of cabbages. PMID: 33203818 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research