CD4+ T Cells cause Renal & Placental Mitochondrial Oxidative Stress as mechanisms of hypertension in response to placental ischemia.

CD4+ T Cells cause Renal & Placental Mitochondrial Oxidative Stress as mechanisms of hypertension in response to placental ischemia. Am J Physiol Renal Physiol. 2020 Nov 16;: Authors: Deer E, Reeve KE, Amaral LM, Vaka VR, Franks M, Campbell N, Fitzgerald S, Herrock OT, Ibrahim T, Cornelius DC, LaMarca BD Abstract The Reduced Uterine Perfusion Pressure (RUPP) rat model and normal pregnant (NP) rat recipients of RUPP CD4+T cells recapitulate many characteristics of preeclampsia (PE) such as hypertension and oxidative stress. We have shown an important hypertensive role for NK cells to cause mitochondrial (mt) dysfunction in RUPP rats, however the role for RUPP CD4+ T cells to stimulate NK cells is unknown. Therefore, we hypothesize that RUPP induced CD4+ T cells activate NK cells to cause mt dysfunction/ROS as mechanisms of hypertension during pregnancy. We tested our hypothesis by adoptive transfer of RUPP CD4+T cells into NP rats or by inhibiting the activation of RUPP CD4+T cells with Orencia (Abatacept) and examining hypertension, NK cells and mt function. RUPP was performed on gestation day 14, splenic CD4+ T cells were isolated on GD19 and injected into NP rats on gestation day (GD) 13. In a separate groups of rats; Orencia was infused and the RUPP procedure performed. MAP and placental and renal mtROS increased in RUPP (n=7, p<0.05) and NP+RUPP CD4+ T cell recipients (n=13, p<0.05) compared to control NP (n=7) and NP+N...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research