Impact of formulary interventions on the minimum inhibitory concentration of methicillin-resistant Staphylococcus aureus to mupirocin, chlorhexidine, and octenidine in a Singapore tertiary institution

AbstractMethicillin-resistantStaphylococcus aureus (MRSA) decolonization is an effective measure to prevent clinical infection but resistance is a concern. We aim to evaluate the impact of mupirocin (MUP) ointment formulary removal, plateauing use of chlorhexidine gluconate (CHG), and hospital-wide introduction of octenidine (OCT)-based products on the minimum inhibitory concentration (MIC) of MRSA to MUP, CHG, and OCT in our hospital. A prevalence study was conducted at three time points (TP) on consecutive MRSA screening isolates to evaluate for their MICs to MUP, CHG, and OCT using broth microdilution sensititre plates and detection of the ileS-2 gene encoding high-level MUP resistance in 2013 (pre-intervention TP1;n = 160), 2016 (early post-intervention TP2;n = 99) and 2017 (late post-intervention TP3;n = 76). Statistical analyses were performed using Chi square test with reference from TP1. There was a significant improvement in MUP susceptibility (MIC< 4 mcg/ml) from 71.9% (TP1) to 86.9% (TP2;p = 0.006) to 88.2% (TP3;p = 0.007). The prevalence of MUP high-level resistance (MIC> 256 mcg/ml) reduced from 25.0% (TP1) to 12.1% (TP2;p = 0.014) to 5.3% (TP3;p = 0.001). Likewise, the prevalence of isolates harboring the ileS-2 gene decreased from 28.1% (TP1) to 18.2% (TP2;p = 0.072) to 9.2% (TP3;p = 0.002). OCT MIC range remains stable at 0.5 to 1 mcg/ml across all three TPs. The proportion of isolates with reduced CHG susceptibility (MIC ≥ 4 mcg/ml) increased over the...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research