Modeling Host-Feeding Preference and Molecular Systematics of Mosquitoes in Different Ecological Niches in Canada.

Modeling Host-Feeding Preference and Molecular Systematics of Mosquitoes in Different Ecological Niches in Canada. Acta Trop. 2020 Nov 04;:105734 Authors: Shahhosseini N, Frederick C, Racine T, Kobinger GP, Wong G Abstract Several mosquito-borne viruses (mobovirus) cause infections in Canada. Ecological data on mosquito species and host range in Canada remains elusive. The main aim of the current study is to determine the host range and molecular systematics of mosquito species in Canada. Mosquitoes were collected using BG-Sentinel traps and aspirators at 10 trapping sites in Canada during 2018 and 2019. Mosquitoes collected were identified via morphology and molecular techniques. Mosquito sequences were aligned by MUSCLE algorithm and evolutionary systematics were drawn using MEGA and SDT software. Moreover, the source of blood meals was identified using a DNA barcoding technique. A total of 5,708 female mosquitoes over 34 different taxa were collected. DNA barcodes and evolutionary tree analysis confirmed the identification of mosquito species in Canada. Of the total collected samples, 201 specimens were blood-fed female mosquitoes in 20 different taxa. Four mosquito species represented about half (51.47%) of all collected blood-fed specimens: Aede cinereus (39 specimens, 19.11%), Aedes triseriatus (23, 11.27%), Culex pipiens (22, 10.78%), and Anopheles punctipennis (21, 10.29%). The most common blood meal sources were humans (49 m...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research