Association between the CEBPA and c-MYC genes expression levels and acute myeloid leukemia pathogenesis and development

AbstractCEBPA andc-MYC genes belong to TF and play an essential role in hematologic malignancies development. Furthermore, these genes also co-regulate withRUNX1 and lead to bone marrow differentiation and may contribute to the leukemic transformation. Understanding the function and full characteristics of  selected genes in the group of patients with AML can be helpful in assessing prognosis, and their usefulness as prognostic factors can be revealed. The aim of the study was to evaluateCEBPA andc-MYC mRNA expression level and to seek their association with demographical and clinical features of AML patients such as: age, gender, FAB classification, mortality or leukemia cell karyotype. Obtained results were also correlated with the expression level of theRUNX gene family. To assess of relative gene expression level the qPCR method was used. The expression levels ofCEBPA andc-MYC gene varied among patients. NeitherCEBPA norc-MYC expression levels differed significantly between women and men (p=0.8325 and p=0.1698, respectively). No statistically significant correlation between age at the time of diagnosis and expression ofCEBPA (p=0.4314) orc-MYC (p=0.9524) was stated. There were no significant associations between relativeCEBPA (p=0.4247) orc-MYC (p=0.4655) expression level and FAB subtype and mortality among the enrolled patients (p=0.5858 and p=0.8437, respectively). However, it was observed thatc-MYC andRUNX1 expression levels were significantly positively correlated (r...
Source: Medical Oncology - Category: Cancer & Oncology Source Type: research