Occludin ‐binding single‐chain variable fragment and antigen‐binding fragment antibodies prevent hepatitis C virus infection

In this study, we converted these mAbs to corresponding monovalent antigen ‐binding fragments (Fabs) and single‐chain variable fragment (scFv) antibodies. These Fab fragments and scFv antibodies demonstrate similar binding specificity and affinity to parental anti‐OCLN mAbs. Moreover, Fab fragments and scFv antibodies inhibitin  vitro HCV infection. The small functional monovalent OCLN ‐binding probes reported in our study have high potential as drug candidates and tools for biological and pharmaceutical studies of OCLN.

Source: FEBS Letters - November 7, 2020 Category: Biochemistry Authors: Yoshimi Shimizu, Takehiro Shinoda, Yoshitaka Shirasago, Masuo Kondoh, Naoko Shinya, Kentaro Hanada, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Tomomi Kimura ‐Someya, Mikako Shirouzu, Masayoshi Fukasawa Tags: Research Article Source Type: research