Cryopreservation of human induced pluripotent stem cells by using a new CryoLogic vitrification method.

In this study, we developed a new friendly CVM by loading a small piece of hiPSCs colonies in the vitrification solution to the hook of Fiberplug to increase the cooling rate. To warm them, the CVM Fiberplug was immersed directly in a 37 °C warming solution for 1 min, and hiPSCs were then transferred to mTeSR1 medium. The result revealed that the new CVM had a high recovery rate and maintained the stemness and differentiation potential of hiPSCs. Our new CVM not only provide a safe way for hiPSCs preservation but also has a high survival rate in the storage of hiPSCs.
PMID: 33147488 [PubMed - as supplied by publisher]

Source: Cryobiology - November 1, 2020 Category: Biology Authors: Zhang Y, Liu H, Liu Z, Long P, Zhao X, Li Q, Huang Y, Ma Y Tags: Cryobiology Source Type: research