Bladder Underactivity Induced by Prolonged Pudendal Afferent Activity in Cats.

Bladder Underactivity Induced by Prolonged Pudendal Afferent Activity in Cats. Am J Physiol Regul Integr Comp Physiol. 2020 Nov 04;: Authors: Mohapatra A, Chen J, Zhao J, Zhong Y, Armann K, Shen B, Wang J, Beckel J, de Groat WC, Tai C Abstract The purpose of this study is to determine the effects of pudendal nerve stimulation (PNS) on reflex bladder activity and develop an animal model of underactive bladder (UAB). In six anesthetized cats, a bladder catheter was inserted via the urethra to infuse saline and measure pressure. A cuff electrode was implanted on the pudendal nerve. After determining the threshold intensity (T) for PNS to induce an anal twitch, PNS (5 Hz, 0.2 ms, 2T or 4T) was applied during cystometrograms (CMGs). PNS (4-6T) of 30-min duration was then applied repeatedly until bladder underactivity was produced. Following stimulation control CMGs were performed over 1.5-2 hours to determine the duration of bladder underactivity. When applied during CMGs, PNS (2T and 4T) significantly (p<0.05) increased bladder capacity while PNS at 4T also significantly (p<0.05) reduced bladder contraction amplitude, duration, and area under contraction curve. Repeated application of 30-min PNS for a cumulative period of 3-8 hours produced bladder underactivity exhibiting a significantly (p<0.05) increased bladder capacity (173±14% of control) and a significantly (p<0.05) reduced contraction amplitude (50±7% of control). T...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - Category: Physiology Authors: Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research