Low cryoprotectant concentration rapid vitrification of mouse oocytes and embryos.

In conclusion, the LCSM protocol, with one medium of much lower cryoprotectant concentrations and shorter equilibration time, reduces exposure to cryoprotectant toxicity while improves efficiency, consistency and reliability for mammalian oocyte and embryo preservation.
PMID: 33137307 [PubMed - as supplied by publisher]

Source: Cryobiology - October 30, 2020 Category: Biology Authors: Liu J, Lee GY, Biggers JD, Toth TL, Toner M Tags: Cryobiology Source Type: research