In Vitro Fermentation and Isolation of Heparin-degrading Bacteria from Human Gut Microbiota.

In Vitro Fermentation and Isolation of Heparin-degrading Bacteria from Human Gut Microbiota. Anaerobe. 2020 Oct 30;:102289 Authors: Pan L, Sun W, Shang Q, Niu Q, Liu C, Li G, Yu G Abstract Heparin and its derivative are commonly used as injectable anticoagulants in clinical, but with poor oral bioavailability. To explore the role of the gut microbiota in the poor oral effect of heparin, the degradation profiles of heparin by six human gut microbiota were investigated. The heparin-degradation ability varied significantly among individuals. Furthermore, two strains of heparin-degrading bacteria, Bacteroides ovatus A2 and Bacteroides cellulosilyticus B19 were isolated from different individual's gut microbiota and the degradation products of the isolates were profiled. The ΔUA2S-GlcNS6S was the major end product, almost no desulfation. 3-O-sulfo group-containing tetrasaccharides were detected, which indicated that the antithrombin binding site was broken and explained the lost anticoagulant activity of heparin. Collectively, the present study assessed the degradation profiles of heparin by human gut microbiota and provided references for the development of oral administration of heparin from a gut microbiota perspective. PMID: 33137435 [PubMed - as supplied by publisher]
Source: Anaerobe - Category: Microbiology Authors: Tags: Anaerobe Source Type: research