Highly Specific Mouse Anti-Joining Chain of Human Immunoglobulin A.

Highly Specific Mouse Anti-Joining Chain of Human Immunoglobulin A. Monoclon Antib Immunodiagn Immunother. 2020 Oct 29;: Authors: Ejemel M, Gawron MA, Schneider MI, Wallace A, Schiller ZA, Schneider R, Martin JC, Klempner MS, Wang Y, Cavacini LA Abstract Immunoglobulin A (IgA) antibodies are critical to mucosal protection, specifically dimeric IgA (dIgA) and secretory IgA (sIgA), which rely on the J chain to polymerize. There is an absence of monoclonal antibodies that can specifically bind to polymeric IgA without the need to denature the molecule. We generated a panel of highly specific mouse anti-J chain antibodies that react with both intact and denatured nonhuman primate dIgA and human dIgA and sIgA of both the IgA1 and IgA2 subclass. We expanded use of this antibody for quantification of dIgA and sIgA using biolayer interferometry or enzyme-linked immunosorbent assay and use for affinity chromatography. This is a significant improvement over available anti-IgA antibodies in the field, which will allow for expanded use in clinical testing. PMID: 33121367 [PubMed - as supplied by publisher]
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research