GSE160229 Targeted high-resolution chromosome conformation capture at genome-wide scale in mouse erythroid cells

Contributors : Damien J Downes ; Jim R HughesSeries Type : OtherOrganism : Mus musculusChromosome conformation capture (3C) provides an adaptable tool through which to study diverse biological questions. Currently, 3C techniques provide either low-resolution interaction profiles across the entire genome, e.g. HiC, or high-resolution interaction profiles at up to several hundred loci, e.g. NG Capture-C and 4C-seq. Generation of high-resolution, genome-wide interaction profiles can feasibly be achieved through efficiency improvements to current high-resolution methods. To this end we systematically tested and removed areas inefficiency in NG Capture-C to develop a new method Nuclear Capture-C, which provides a 300% increase in informative sequencing content. Using Nuclear Capture-C we target 8,026 erythroid promoters in triplicate, showing that this method can achieve high-resolution genome-wide 3C interaction profiles at scale.

Source: GEO: Gene Expression Omnibus - October 28, 2020 Category: Genetics & Stem Cells Tags: Other Mus musculus Source Type: research