GSE146128 Functional Dissection of Transcription Factor Networks Governing Haematopoietic Progenitor States

Contributors : Iwo Kucinski ; Nicola K Wilson ; Sarah J Kinston ; Rebecca Hannah ; Berthold GottgensSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusBlood is generated by a constant stream of differentiating haematopoietic progenitor cells. The process is controlled by an immensely complex gene regulatory networks. These have been difficult to comprehend using correlative evidence and limited systematic functional data. Hoxb8-FL cell line is a model system of lympho-myeloid progenitors, which self-renews in vitro and is amenable to genetic perturbations. To construct a functionally defined transcription factor (TF) network we targeted 39 transcription factors using CRISPR/Cas9 gene targeting in Hoxb8-FL cells. We measured the resulting transcriptional changes by small scale RNA-Seq within 2-4 d of each perturbation. Our network analysis revealed>17,000 TF-target interactions across>7,000 target genes, established new interactions among TFs and shed new light on the mechanisms maintaining self-renewal and multipotency.

Source: GEO: Gene Expression Omnibus - October 26, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research