GSE159308 Gene expression profile of Cxcl12-DsRed+ stromal cells from E15.5, P5, and P10 livers.

Contributors : Lei Ding ; Yeojin LeeSeries Type : Expression profiling by arrayOrganism : Mus musculusThe study is designed to identify transcriptional differences of CD45-Ter119-Cxcl12-DsRed+ stromal cells at different develomental stages. 50,000 CD45-Ter119-Cxcl12-DsRed+ stromal cells from freshly prepared livers of E15.5, P5 and P10 Cxcl12DsRed/+ mice were sorted into Trizol by flow cytometry. Total RNA was isolated and amplified with the WT-Ovation Pico RNA Amplification system (Nugen) as the manufacturer ’s instructions. Sense strand complementary DNA was generated using the WT-Ovation Exon Module (Nugen). Then, cDNA was fragmented and labelled using FL-Ovation DNA Biotin Module V2 (Nugen). The labelled cDNA was hybridized to Affymetrix Mouse Gene ST 1.0 chips following the manufacturer's instruct ions.

Source: GEO: Gene Expression Omnibus - October 26, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research