Site-specific chemical fatty-acylation for gain-of-function analysis of protein S-palmitoylation in live cells.

Site-specific chemical fatty-acylation for gain-of-function analysis of protein S-palmitoylation in live cells.
Chem Commun (Camb). 2020 Oct 23;:
Authors: Li Y, Wang S, Chen Y, Li M, Dong X, Hang HC, Peng T
Abstract
Protein S-palmitoylation, or S-fatty-acylation, regulates many fundamental cellular processes in eukaryotes. Herein, we present a chemical fatty-acylation approach that involves site-specific incorporation of cycloalkyne-containing unnatural amino acids and subsequent bioorthogonal reactions with fatty-acyl tetrazines to install fatty-acylation mimics at target protein sites, allowing gain-of-function analysis of S-palmitoylation in live cells.
PMID: 33094750 [PubMed - as supplied by publisher]

Source: Chemical Communications - October 23, 2020 Category: Chemistry Authors: Li Y, Wang S, Chen Y, Li M, Dong X, Hang HC, Peng T Tags: Chem Commun (Camb) Source Type: research