Photoactivatable Fluorophore for STED Microscopy and Bioconjugation Technique for Hydrophobic Labels.

Photoactivatable Fluorophore for STED Microscopy and Bioconjugation Technique for Hydrophobic Labels.
Chemistry. 2020 Oct 23;:
Authors: Hell SW, Weber M, Khan TA, Patalag LJ, Bossi M, Leutenegger M, Belov VN
Abstract
The use of photoactivatable dyes in STED microscopy has so far been limited by two-photon activation through the STED beam and by the fact that photoactivatable dyes are poorly solvable in water. Here we report ONB-2SiR, a fluorophore that can be both photoactivated in the UV and specifically de-excited by STED at 775 nm. Likewise, we introduce a conjugation and purification protocol to effectively label primary and secondary antibodies with moderately water-soluble dyes. Greatly reducing dye aggregation, our technique provides a defined and tunable degree of labeling, and improves the imaging performance of dye conjugates in general.
PMID: 33095954 [PubMed - as supplied by publisher]

Source: Chemistry - October 23, 2020 Category: Chemistry Authors: Hell SW, Weber M, Khan TA, Patalag LJ, Bossi M, Leutenegger M, Belov VN Tags: Chemistry Source Type: research